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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16001" title="Hi, I am new in the field of epigenetics and need some help to understand primer design in methylation studies.I have tried the MSP-Primer and Methprimer programmes but when I want to check the primer-position (especially with MSPPrimer) I never find the ..." target="_self">Primer Design MSP, BSP, MS-HRM</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15977" title="Hi all, I have been trying get amplification from my bisulfite treated gDNA, but have had no luck so far and would really appreciate any help.I am trying to assess the methylation status of a transgene by bisulfite sequencing. I have several primer sets t..." target="_self">Bisulfite sequencing not working!</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15939" title="Hey what are the imp criteria to consider while setting up a PCR reaction using bisulphite modified DNA)(Im trying to do bisulphite sequencing)..." target="_self">bisulphite sequencing</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15923" title="Hello everyone,I found this message board when searching on google for information on epigenetics and methylation, after a theory was posted on a support group for people who have been physically damaged by the drug finasteride (propecia) even long after ..." target="_self">Not a Geneticist - But looking for assistance - please read</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15875" title="Hello everyone!I need help, my MS-PCR (Met) is amplifying unmodified DNA. Using previously described primers. How can?Thanks..." target="_self">MSP - unwanted amplification</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15868" title="Hi everybody,I have a smear problem in my Bisulfite PCR. I have done this PCR with same primers, it was giving a band but it was also giving some smear in gel. But now only thing i can get is smear, i have changed buffer, Mg, primers, PCR water but i stil..." target="_self">Smears in Bisulfite seq. PCR</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15852" title="To analyze if the promoters of a gene (in which we are interested) are methylated the genomic DNA was digested.Then, the DNA (2ug) was bisulphited following a standard protocol (16 hours at 55 ° C). The bisulphited DNA was cleaned, precipitated, purified ..." target="_self">DNA bisulphited Amplification</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15833" title="Hi,I have recently started studying DNA methylation. I have used the Qiagen EpiTech kit for bisulfite conversion.I start with 500ng to 1ug Genomic DNA. For bisulfite PCR analysis I have used primers designed by the Methyl Express software amplfying around..." target="_self">Multiple bands after bisulfite PCR</a><br />');
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