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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16014" title="hii am measuring superoxides in chlorella cells with dihydroethidium (DHE). Using a final concentration of 30 micromolar DHE (sigma) working stock in MeOH for 100000 cells per ml washed and suspended in PBS, I incubated for 15 min in dark at 37 C with sha..." target="_self">slit widths for Dihydroethidium Fluoresence in Perkin elmer LS 55 Fl Spec</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15774" title="hello,I wanna look at the cytokine profile of the DCs form the lungs of the lungs of the mice that received a viral infection followed by a bacterial infection. I think I can do surface staining of the cells followed by I/C staining. Can i treat the cells..." target="_self">Intracellular staining for Cytokines for cells stimulated in vivo</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15682" title="Hello everybody,I am novice to cell cycle anaysis and done this procedure ( Propidium iodide staining) for the first time in A431 cancer cell lines.my results were discouraging the person who analysed my samples for cell cycle analysis told me that the ce..." target="_self">problem with cell cycle analysis</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15467" title="Hi all,I\'ve recently been to a congress in which several flow cytometers were exposed.As you can imagine, every business agent said that his cytometer was better than others...Since I\'m not an expert in this field, could anyone tell me what are the feat..." target="_self">How to choose a flow cytometer</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15453" title="Hi,I am growing MCF-7 cell line. I have been trying to stain dead cells using ethidium bromide and I havnt been very successful. I found the protocol online but it doesnt seem to work. if there is any other method with which I can stain the cells please l..." target="_self">Ethidium bromide protocol</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15398" title="hi. I am new to this field...i wanted to know where the cell cycle is arrest (Go/G1, S/G2, M) is in mutant mice.i just want to ask if its possible to directly get pancreas samples from mice injected with BrDU and isolate tissue and collect the cells direc..." target="_self">cell cycle analysis from mice tissue</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15276" title="i am currently not that familiar with FACS analysis:is it possible to test the same sample for apoptosis AND proliferation markers?could it be that there is a double positive population - e.g. apoptotic cells which express proliferation markers or vice ve..." target="_self">FACS - double staining</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15153" title="hi everyone,we wanna do qpcr with lymphocytes isolated from cat blood. we isolate pbmc by density gradient centrifugation on ficoll and label them with mab. our technician sorts them into 3 different populations (using the FACSaria) and we extract rna rig..." target="_self">cell sorting - losing cells?</a><br />');
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