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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16095" title="Hello everybody,I am trying to stain peritoneal macrophages of mouse with Oil Red O. I did it once with an Oil Red O prepared 0,2% in Methanol 100% and NaOH 1M (0,07g Oil Red O, 25ml Mehanol, 10ml NaOH 1M), and everything was fine. The problem is now, eve..." target="_self">Stainning of peritoneal macrophages with Oil Red O</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16076" title="Hi, does anybody know why some of my cortical neurons are black? Maybe the dissection is not very fair and I took some neurons of other brain regions?I have a contamination of erythrocytes too, i hope i could eliminate them with changing medium...Thanks..." target="_self">cortex neuronal culture</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16071" title="HiI need to do some elisa on my CHO cell line, is there any suitable cell lysis buffer that I can prepare in lab?regards..." target="_self">suitable cell lysis buffer</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16024" title="i thawed out some hek293 cells and plated them and they have been growing for about 3 days. some have attached and are growing in groups. however, they dont look like normal hek293 should look (according to pictures). my cells look more round and are grow..." target="_self">Thaw HEK293</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16023" title="Hi Everyone,I am in great need of assistance regarding some contamination in our cell culture room. I am in the final months of my research of my PhD and now am having some huge problemsRecently, I have started sharing the tissue culture room with some ne..." target="_self">Aspergillus in cell culture room - HELP!!! how hardcore to decontami</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16002" title="1.How much medium should be added into the conical flask and how long can the plant survives in the environment without depletion of medium?2.What are the challenges that you might encounter when culturing the in-vitro plants? 3.How the in-vitro plant can..." target="_self">Simply question about plant cell culture</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15992" title="hiI am new in cell culture and I am confused about correct protocol for cell counting using a hemocytometer, actually in our lab some people use the central square of hemocytometer for counting and others count four side squares and divide it to 4. which ..." target="_self">very simple question about hemocytometer cell counting</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15980" title="Hi, I would like to culture cells A549 and MH-S in vitro with cigarette smoke extract (CSE), but I do not have a good protocol to prepare CSE. If anybody could help me and pass me a good one...it would be of great help for me. thanks so much!..." target="_self">cigarette smoke extract</a><br />');
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