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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16133" title="Hi I need a protocol for clear native NAGE. i need it to perform in-gel activity asay for electron transport chain complex lll. Also if anyone could help me with providing a protocol for this activity assay..." target="_self">Clear native PAGE</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16127" title="Hi....I am having a major problem with my western blotting. I am purifying small amounts of a his tagged recombinant protein. I just recently ran a gel and transfered it to PVDF. I confirmed transfer with Ponceu S. Here\'s were the problem occurs. We boug..." target="_self">help my proteins are disappearing from my PVDF membranes</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16103" title="Hi everyone,I am extracting protein from endothelial cells using a cytoplasmic/nuclear extraction kit from Cayman Chemical (http://www.caymanchem.com/app/template/Product.vm/catalog/10009277/a/z). The protocol provided by the manufacturer is followed exac..." target="_self">No bands in cytosolic extraction, nice bands in nuclear extraction--see pics</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16092" title="I performed a western blot that didnt work.I got the gel working ok, and Ponceau staining showed that the transfer worked perfectly.I blocked the membrane in 5% BSA in 0.1% TBS-Tween for 1 hour.Then i washed it for 5 minutes in 6mls of 0.1% TBS-Tween. I d..." target="_self">Western not working anymore</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16083" title="Hi everyone,I am a newbie in WB and was trying to analyse the expression of Nav1.8 sodium channel from rat DRG lysates. I purchased the Anti-Nav1.8 Ab from the Alomone Labs Ltd. (Jerusalem, Israel) as I found quite a number of papers trying to use their p..." target="_self">Problem regarding Anti-Na+ Channel Ab</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16028" title="Hiya!Hope everyone is well.. I just have a quick question and any response would be greatly appreciated!! .. I am in the process of expressing a fickly flagellin protein on a larger scale to get antibodies. We have cloned into PgMET and then into PQE-Xa 3..." target="_self">Help!!! Having issues expressing protein on a large scale</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16022" title="Whats the difference between SDS and RIPA for cell lysis?I have been using SDS (very strong detergent) that lyses everything, and i do not need to spin down to get cell debris pellet.But RIPA i seen my colleagues use, they have to spin down, get rid of th..." target="_self">SDS vs RIPA for cell lysis</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16005" title="Hi all, just wondering if anybody knows a protocol for BN-PAGE using Tris-glycine system? All the protocols and papers i read on BN-Page uses Bis-Tris tricine system with pH ~7. If I use a tris-glycine system, should i adjust my pH to 7 as well? Thanks..." target="_self">BN-PAGE possible with Tris-glycine</a><br />');
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