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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16041" title="Dear colleagues,as there are 9 human Importin alpha subtypes (alpha1, alpha2, ...) I was wondering if there are siRNAs which can knockdown all subtypes simultaniously. There are two regions 100 % conserved in alle 9 Importin alphas. Have you ever seen suc..." target="_self">Importin alpha siRNA</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16032" title="Hello everyoneI want to clone the UTR of my gene of interest in one expression vector, downstream of luciferase or GFP gene to access if it\'s controlled by miRs. Since after a lot of attempts I couldn’t clone my UTR my boss opened the wallet and said tha..." target="_self">miRNA 3´UTR target expression vectors</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15987" title="Hi,I\'m using two systems trying to get knockdown of gene the one is the pSUPER RETRO and the other is the tet on/offI made stables cell line in both system selecting with antibiotic, somehow when I\'m doing real time PCR I\'m not getting knock down. the ..." target="_self">shRNA</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15965" title="Hi all!I want to perfom gene silencing on cells seeded in a 24-well plate. I would like to control by semi-quantitative pcr the expression of the transcript of interest, but I\'m afraid that cells in a well are not enough to perform acid nucleic extractio..." target="_self">nucleic acid extraction from silenced cells</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15953" title="Hi,I\'d like to know if there is any miRNA that is located in an exon of a gene. Is there any plant miRNA that is located in an exon of a gene? Almost all papers state that miRNAs are located within intron or intergenic. Could anyone there answer this que..." target="_self">Is there any pre-miRNA located in exon?</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15795" title="Hello,I have a question about the different packaging kits for lentiviral particle production sold by the various companies. At the moment I am looking to buy shRNA lentiviral plasmid to target my gene of interest. I\'ve found a possible option on genecop..." target="_self">Differences between lentiviral packaging kits</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15787" title="Hey guys!I am having a problem in my cell culture. Everyother time I infect cells with a given shRNA some of the plates I infected get contaminated. They became smelly and with a cloudy/white medium. I would say it is a bacterial contamination (it is too ..." target="_self">Contamination after shRNA infection</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15654" title="Hi everyone,I\'m currently trying to plan an experiment which will involve human miRNA microarray analysis but currently I don\'t have too much experience in these procedures. Does anyone have suggestions of methods/ kits to create a cDNA library/ amplify..." target="_self">miRNA microarray</a><br />');
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