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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16120" title="Hi all,I have brain tissue that I\'m trying to extract RNA from. I added 2 mL of Trizol and 400 uL of chloroform, vortexed, and centrifuged at 4C for 15 mins. After centrifugation, I could not detect an interphase layer in the tube; all I saw was a top aq..." target="_self">problem with Trizol RNA extraction</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16110" title="Hi,I\'ve never done site-directed mutagenesis before and have purchased pfu turbo taq pol (Stratagene) and DpnI and primers. I\'m trying to follow the QuikChange Site-Directed Mutagenesis manual and have come to a bit of a stopping point. This might sound..." target="_self">Mutagenesis</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16087" title="hey people can anyone guide me to some site wherein i can put my DNA sequence and get the protein sequence??!!!thanx!!..." target="_self">Sites for protein sequence from DNA sequence</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16084" title="hi,last time I centrifuged my protein G at 2400rpm. but Sigma protocol for my new anti-flag slurry gel suggests over 9000rpm. isn\'t this too high to break the beads and release bound antibodies?..." target="_self">will high-speed centrifuge rupture protein G?</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16081" title="Hello all,I did a stupid mistake today and am thinking what to do except waiting for the gel pic what i will get after 2 days.I use phenol chloroform to extract DNA from cells. So i treat the cells with extraction buffer and it has rnase a. After that i t..." target="_self">rnase treatment</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16050" title="solved..." target="_self">CRIM Integration</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16046" title="we want to enrich carcinoma cells from buffy coat. after cell preparation i want to isolate RNA - it is known that there are only 1 carcinoma cell in 1000.000 leukocytes (at least i have around 100 cells used for RNA isolation.does anyone of you know a pr..." target="_self">RNA Isolation of MACS sorted cells</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16038" title="Has anyone ever used this? Is it any better than bleach?..." target="_self">DNA zap</a><br />');
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