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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=16059" title="Hi all out thereI hope some one can help me outI\'m currently validating hits from an array study to se if they are true targets of my protein (a transcription factor) or not. My PI want me to conduct a ChiP assay only focusing on the most primary candida..." target="_self">How to design primers to check my candidates in a ChiP assay</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15878" title="Hi, I am new to this forum and new to ChIP...two weeks ago, i did a ChIP with my bossthe result showed no PCR product from the ChIP, but input doesit was with 1 well of ES cells from a 6-well plate, using bioruptor for sonication, 1ml solutionthen I went ..." target="_self">no PCR product from input...</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15727" title="Dear ChIP-friends,I am facing a big problem. The HeLa cells which I am using for ChIP do not lyse in SDS buffer anymore after they were infected with adenovirus. No lysis method used is able to break the cells, not even protein sample (laemmli-buffer). Ha..." target="_self">Lysis of the cells</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15724" title="Dear ChIP-friends,I am facing a big problem. I do ChIP Assays with HeLa cells which are infected with Adenovirus. Recently, the problem arose that the infected HeLa cells do not lyse in SDS-buffer anymore. They aggregate in kind of colonies and no lysis m..." target="_self">Cells do not lyse in SDS buffer</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15243" title="Happy Friday everyone!We have hit a major snag in analysing our chip-chip data. Basically, I have done chip-chip on 59 patients (2 histone mods each) and 15 controls - so that\'s a HUGE dataset! What we would really like to do is cluster our data (we are ..." target="_self">ChIP-chip - scaling data in huge datasets</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15102" title="Hi, Colleagues,For the ChIP agarose gel results, should the input always be more than the IP one? Please see the figure. With my understanding, input group includes the global DNA fragments that are PCRed, but IP group only contains the DNA that bound the..." target="_self">the ChIP agarose gel results</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15086" title="Dear colleagues,I want to find binding sites of my transcription factor via ChIP-seq. I have a polyclonal antibody that weakly recognizes my protein in western (besides numerous other bands) and I am not sure if I can use that antibody for ChIP. How could..." target="_self">ChIP antibody question</a><br />');
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document.write('<li class="rss-item"><a class="rss-item" href="http://www.protocol-online.org/forums/index.php?showtopic=15037" title="Hello,I currently use Chelax and then incubate for 55 deg., followed by 95 deg. I understand the 95 deg. incubation to denature the DNA into single strands. Am I still able to quantify and detect using picogreen on a fluorimeter the amount of ChIP DNA in ..." target="_self">Chelax and Quantification</a><br />');
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